Percutaneous Epididymal Sperm Aspiration (PESA)
Percutaneous epididymal sperm aspiration (PESA), is a method where sperm is collected from the epididymis inside the scrotum using a syringe and fine needle. The epididymis is a narrow, tightly-coiled tube that is attached to each of the testicles and is where sperm cells mature and are stored. The sperm is looked at under a microscope in a laboratory, where it may be used right away to fertilize eggs or frozen for future infertility treatment.
This method is often used when there is no sperm found in the ejaculate due to hindrance in the passages. Reasons for this include vasectomy reversal, damage to the vas deferens from infection, or bilateral congenital absence of vas deferens.